Difference between revisions of "Part:BBa K165006:Design"
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+ | Reynolds L., Ullman C., Moore M., Isalan M., West M.J., Clapham P., Klug A., Choo Y. 2003. Repression of the HIV-1 5LTR promoter and inhibition of HIV-1 replication by using engineered zinc-finger transcription factors. PNAS 2003 (100) 4:1615–1620 |
Revision as of 17:38, 26 October 2010
Zif268-HIV DNA-binding domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 73
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
[http://2010.igem.org/Team:Slovenia/ Team Slovenia 2010] deposited a modified variant of HivC intended for easier isolation, purification and other experiments. We added HIS tag to C-terminal side of HivC sequence and C-terminal split CFP on N-terminal part of HivC after an extension linker. We deposited modified variant of HivC as cCFP_link_HivC BBa_K323071 and additionally characterized it with several methods.
Source
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References
Reynolds L., Ullman C., Moore M., Isalan M., West M.J., Clapham P., Klug A., Choo Y. 2003. Repression of the HIV-1 5LTR promoter and inhibition of HIV-1 replication by using engineered zinc-finger transcription factors. PNAS 2003 (100) 4:1615–1620