Difference between revisions of "Part:BBa K404122"
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[[Image:Freiburg10_Vectorplasmid composite 4.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_Vectorplasmid composite 4.png|thumb|center|480px]]<br>
 
<h2>ITRs</h2><br>
 
<h2>ITRs</h2><br>
 +
[[Image:Freiburg10_ITR.png|thumb|center|480px]]<br>
 +
The inverted terminal repeat structures can be subdivided into several palindromic motives: A and A’ form a stem loop which encases B and B’ as well as C and C’. Those motives form both arms of the T-shaped structure. The functional motives on the ITR are two regions that bind Rep 68/78, called Rep-binding elements (RBE on the stem and RBE’ on the B arm) and the terminal resolution site (trs) in which the rep proteins introduce single-stranded nicks. The 3’ OH end of the A motive acts as a primer for DNA replication (Im & Muzyczka, 1990) (Lusby, Fife, & Berns, 1980).
 
<h2>CMV promoter</h2><br>
 
<h2>CMV promoter</h2><br>
 
<h2>Beta-globin-intron</h2><br>
 
<h2>Beta-globin-intron</h2><br>

Revision as of 16:47, 26 October 2010

[AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR

Freiburg10 Vectorplasmid composite 4.png

ITRs


Freiburg10 ITR.png

The inverted terminal repeat structures can be subdivided into several palindromic motives: A and A’ form a stem loop which encases B and B’ as well as C and C’. Those motives form both arms of the T-shaped structure. The functional motives on the ITR are two regions that bind Rep 68/78, called Rep-binding elements (RBE on the stem and RBE’ on the B arm) and the terminal resolution site (trs) in which the rep proteins introduce single-stranded nicks. The 3’ OH end of the A motive acts as a primer for DNA replication (Im & Muzyczka, 1990) (Lusby, Fife, & Berns, 1980).

CMV promoter


Beta-globin-intron


mGMK_TK30 fusion protein


hGH


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 1977
    Illegal NgoMIV site found at 2882
    Illegal AgeI site found at 3011
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3413
    Illegal SapI.rc site found at 1369