Difference between revisions of "Part:BBa K404205"

(Restriction sites)
Line 10: Line 10:
 
===Restriction sites===
 
===Restriction sites===
  
</html><table border="1" style="float:right; width:480px; height:auto;>
+
<table border="1" style="float:right; width:480px; height:auto;>
 
  <tr>
 
  <tr>
 
   <th><b>Restriction site</b></th>
 
   <th><b>Restriction site</b></th>

Revision as of 15:51, 26 October 2010

ViralBrick-587-BAP
The Biotinylation Acceptor peptide motif, ready for insertion into the 587 loop

Freiburg10 ViralBrick-logo-587-BAP.png

Usage and Biology

Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 587 [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] Freiburg10 recognition site BamHI-HF.Gif 100;50;10;100 37°C
downstream 587 [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] Freiburg10 recognition site PvuII-HF.Gif 0;25;0;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]