Difference between revisions of "Part:BBa K380006:Design"

 
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<partinfo>BBa_K380006 short</partinfo>
 
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Human bFGF-GST in pGEX was kindly provided by Nozomu Nishi, Kagawa University, Japan [1].
 
Human bFGF-GST in pGEX was kindly provided by Nozomu Nishi, Kagawa University, Japan [1].
  
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[1] Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain
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[1] Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain, 1998
  
 
===References===
 
===References===

Latest revision as of 17:35, 27 October 2010

Human basic fibroblast growth factor, bFGF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 403
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


Design Notes

An AgeI restriction site was situated at nt 340-345. As assembly standard 25 was used the restriction site was removed with site-directed mutagenesis, by changing nt 341 from C to T.


Source

Human bFGF-GST in pGEX was kindly provided by Nozomu Nishi, Kagawa University, Japan [1].

[1] Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain, 1998

References