Difference between revisions of "Part:BBa K338004"
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− | This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part | + | This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part <partinfo>BBa_K338003</partinfo>. |
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Revision as of 11:14, 26 October 2010
PHA Synthase Composite, Part 2/2
This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains phaB1 and phaC1: BBa_K156013 & BBa_K156014. It was designed to be ligated downstream of part BBa_K338003.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1348
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 697
Illegal AgeI site found at 1047 - 1000COMPATIBLE WITH RFC[1000]