Difference between revisions of "Part:BBa K346033"
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Figure 1: The structure of Dsba+MBP. This protein's function is to carry MBP to the periplasm and bind heavy metal there. | Figure 1: The structure of Dsba+MBP. This protein's function is to carry MBP to the periplasm and bind heavy metal there. | ||
| − | [[Image: | + | [[Image:dfadsfds.jpg]] |
Figure 2: The expression of Dsba+MBP. We can see that the 40 kd band both in the SDS-PAGE and Western blotting result. | Figure 2: The expression of Dsba+MBP. We can see that the 40 kd band both in the SDS-PAGE and Western blotting result. | ||
Latest revision as of 07:07, 26 October 2010
T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)
This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide)
Description
DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .
Figure 1: The structure of Dsba+MBP. This protein's function is to carry MBP to the periplasm and bind heavy metal there.
Figure 2: The expression of Dsba+MBP. We can see that the 40 kd band both in the SDS-PAGE and Western blotting result.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 529
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 529
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 317
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 529
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 529
Illegal AgeI site found at 149 - 1000COMPATIBLE WITH RFC[1000]