Difference between revisions of "Part:BBa K346019"

Latest revision as of 05:22, 26 October 2010

PmerT promoter(03)+RBS(B0034)+lacZ alpha(BBa_I732006)

Compared to BBa_K346016, this part use lacZ alpha as a reporter instead of GFP. When treated with X-gal, the strain's color would be an indication for the concentration of Hg(II).Compared to BBa_K346017, PmerT was replaced by its mutant 3 to make strain with different sensitivity to Hg(II).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 05:14, 26 October 2010 (view source) Kitties (Talk \| contribs) ← Older edit		Latest revision as of 05:22, 26 October 2010 (view source) Kitties (Talk \| contribs)
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	<partinfo>BBa_K346019 short</partinfo>		<partinfo>BBa_K346019 short</partinfo>

−	Compared to BBa_K346016, this part use lacZ alpha as a reporter instead of GFP. When treated with X-gal, the strain's color would be an indication for the concentration of Hg(II).Compared to BBa_K346017, PmerT was replaced by its mutant 88 to make strain with different sensitivity to Hg(II).	+	Compared to BBa_K346016, this part use lacZ alpha as a reporter instead of GFP. When treated with X-gal, the strain's color would be an indication for the concentration of Hg(II).Compared to BBa_K346017, PmerT was replaced by its mutant 3 to make strain with different sensitivity to Hg(II).