Difference between revisions of "Part:BBa K349002"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
  
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This plasmid is used to create BA linker style parts for the BioByte Assembly Method v.2.  The RFP coding cassette provides a selection method for your parts.  
+
This plasmid is used to create BA linker style parts for the BioBytes Assembly Method 2.0.  The RFP coding cassette provides a selection method for your parts.  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 01:00, 27 October 2010

RFP-BA-BsaI (for construction of BA BioBytes 2.0)

Base Plasmid v.2 for the construction of new BioBytes-v2. The RFP cassette can be removed using the BsaI enzyme and a new BA linker style part may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly System-v2.


Usage and Biology

This plasmid is used to create BA linker style parts for the BioBytes Assembly Method 2.0. The RFP coding cassette provides a selection method for your parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086