Difference between revisions of "Part:BBa K349117:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. NOTE: this part is in a pSB1A3 backbone. | + | This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. . Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method. NOTE: this part is in a pSB1A3 backbone. |
===Source=== | ===Source=== |
Revision as of 01:15, 27 October 2010
tetR-AB (BioBytes 2.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1300
Design Notes
This part has built-in BsaI cutsites surrounding a BBa_P1005 tetracycline antibiotic resistance cassette. . Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method. NOTE: this part is in a pSB1A3 backbone.
Source
pSB1A3