Difference between revisions of "Part:BBa K349001"
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<partinfo>BBa_K349001 short</partinfo>
 
<partinfo>BBa_K349001 short</partinfo>
  
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Base plasmid v.2 for the construction of BioBytes-v2.  The RFP cassette can be removed using the BsaI enzyme and a new AB ORF style part may be inserted into the plasmid for amplification.
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Base plasmid v.2 for the construction of components of the BioBytes 2.0 assembly method.  The RFP cassette can be removed using the BsaI enzyme and a new AB BioByte may be inserted into the plasmid for amplification.
  
  
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This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.
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This plasmid is used to create AB parts for the BioBytes 2.0 assembly method.
  
  

Revision as of 00:57, 27 October 2010

RFP-AB-BsaI (for construction of AB BioBytes 2.0)

Base plasmid v.2 for the construction of components of the BioBytes 2.0 assembly method. The RFP cassette can be removed using the BsaI enzyme and a new AB BioByte may be inserted into the plasmid for amplification.


Usage and Biology

This plasmid is used to create AB parts for the BioBytes 2.0 assembly method.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086