Difference between revisions of "Part:BBa K302034"
Line 2: Line 2:
 
<partinfo>BBa_K302034 short</partinfo>
 
<partinfo>BBa_K302034 short</partinfo>
  
−
Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus Subtilis''. These genes are use in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.
+
Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus Subtilis''. These genes are used in ''Bacillus Subtilis'' to provide a toxin-antitoxin kill switch in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:31, 26 October 2010

mazEF toxin-antitoxin cluster

Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus Subtilis. These genes are used in Bacillus Subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]