Difference between revisions of "Part:BBa K302031:Design"
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Sequence selected based on the work of Shimotsu and Henner in the paper below
+
Sequence selected based on the work of Steinmetz et al. and Meng and Futterer in the papers below
  
 
===Source===
 
===Source===
  
−
''Bacillus Subtilis'' 168 strain
+
''Bacillus subtilis'' 168 strain
  
 
===References===
 
===References===
  
−
Shimotsu, H., and Henner, D. J. (1986) ''J. Bacteriol.'' 168, 380–388
+
Steinmetz, M., Le Coq, D., Aymerich, S., Gonzy-Treboul, G. &
 +
Gay, P. (1985). The DNA sequence of the gene for the secreted
 +
''Bacillus subtilis enzyme'' levansucrase and its genetic control sites. ''Mol
 +
Gen Genet'' 200, 220–228.
 +
 
 +
Meng, G. & Futterer, K. (2003). Structural framework of fructosyl
 +
transfer in ''Bacillus subtilis'' levansucrase. ''Nat Struct Biol'' 10, 935–941.

Revision as of 01:29, 26 October 2010

Sucrose sensitive inducer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence selected based on the work of Steinmetz et al. and Meng and Futterer in the papers below

Source

Bacillus subtilis 168 strain

References

Steinmetz, M., Le Coq, D., Aymerich, S., Gonzy-Treboul, G. & Gay, P. (1985). The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol Gen Genet 200, 220–228.

Meng, G. & Futterer, K. (2003). Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat Struct Biol 10, 935–941.