Difference between revisions of "Part:BBa K274003:Experience"

Revision as of 15:27, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274003

User Reviews

E. coli turned dark green after transformation of this plasmid. This is unexpected as there is no promoter upstream of the operon.

BBa_K274003 Review No.2 UCL_London, Xiang Chen

Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below.

This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.

UNIQa38cefd9d8759fb5-partinfo-00000000-QINU UNIQa38cefd9d8759fb5-partinfo-00000001-QINU

@@ Line 7: / Line 7: @@
 ===User Reviews===
-E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
+E. coli turned dark green after transformation of this plasmid. This is unexpected as there is no promoter upstream of the operon.
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@@ Line 15: / Line 15: @@
 <I>UCL_London, Xiang Chen</I>
 |width='60%' valign='top'|
-There is no XbaI restriction site in this plasmid, thus cannot be used to asseble after other parts.
 Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below.
+This plasmid did not cut with Xba I restriction site in our hands. As such we could not  use it to assemble after other parts.
 [[Image:K274003 Diagnostic Gel .jpg|center|300px]]