Difference between revisions of "Part:BBa K302012:Experience"
RachelBoyd (Talk | contribs) (→Applications of BBa_K302012) |
RachelBoyd (Talk | contribs) (→Applications of BBa_K302012) |
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|[[Image:Teamnewcastle_yneA168.png|800px]] | |[[Image:Teamnewcastle_yneA168.png|800px]] | ||
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| + | |Graph 1 shows that overexpression of the ''yneA'' gene (Δ''amyE'':pSpac(hy)-oid::''yneA'') leads to a longer cell length compared with our control ''Bacillus subtilis 168''. | ||
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|'''See below''': ''Bacillus subtilis 168'' cells (left),''Bacillus subtilis'' expressing ''yneA''(centre) and ''Bacillus subtilis'' overexpressing ''yneA''(right) | |'''See below''': ''Bacillus subtilis 168'' cells (left),''Bacillus subtilis'' expressing ''yneA''(centre) and ''Bacillus subtilis'' overexpressing ''yneA''(right) | ||
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|[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_yneA1.jpg|300px]][[Image:Teamnewcastle_yneA.jpg|300px]] | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_yneA1.jpg|300px]][[Image:Teamnewcastle_yneA.jpg|300px]] | ||
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|'''Graph2''': | |'''Graph2''': | ||
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|[[Image:newcastle_no induction.jpg|600px]] | |[[Image:newcastle_no induction.jpg|600px]] | ||
| + | |- | ||
| + | |Graph 2 shows the percentage of cells at different lengths(μm)uninduced | ||
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|'''See below''': ''Bacillus subtilis 168'' cells (left) and non-induced cells(right) | |'''See below''': ''Bacillus subtilis 168'' cells (left) and non-induced cells(right) | ||
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| − | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_noindBS.jpg|300px]] | + | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_noindBS.jpg|300px]] |
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|- | |- | ||
|'''Graph3''': | |'''Graph3''': | ||
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|[[Image:newcastle_0.2 induction.jpg|600px]] | |[[Image:newcastle_0.2 induction.jpg|600px]] | ||
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| + | |Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG | ||
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|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 0.2mM IPTG(right) | |'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 0.2mM IPTG(right) | ||
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|[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_0.2indBS.jpg|300px]] | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_0.2indBS.jpg|300px]] | ||
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|- | |- | ||
|'''Graph4''': | |'''Graph4''': | ||
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|[[Image:newcastle_1IPTG.jpg|600px]] | |[[Image:newcastle_1IPTG.jpg|600px]] | ||
| + | |- | ||
| + | |Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG | ||
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|'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 1mM IPTG(right) | |'''See below''': ''Bacillus subtilis 168'' cells (left) and cells induced at 1mM IPTG(right) | ||
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| − | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_1indBS2.jpg|300px]] | + | |[[Image:Teamnewcastle_yneA168BS.jpg|300px]][[Image:Teamnewcastle_1indBS2.jpg|300px]] |
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|} | |} | ||
Revision as of 22:08, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K302012
| Graph1: |
|
| Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168. |
| See below: Bacillus subtilis 168 cells (left),Bacillus subtilis expressing yneA(centre) and Bacillus subtilis overexpressing yneA(right) |
  
|
| Graph2: |
|
| Graph 2 shows the percentage of cells at different lengths(μm)uninduced |
| See below: Bacillus subtilis 168 cells (left) and non-induced cells(right) |
|
| Graph3: |
|
| Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right) |
|
| Graph4: |
|
| Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right) |
|
Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.
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