Difference between revisions of "Part:BBa K364318:Design"
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===Source=== | ===Source=== | ||
− | C. elegans orphan nuclear receptor | + | C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-80 placed into pSB1C3 carrier vector. |
===References=== | ===References=== | ||
1. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. "Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans." [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDG-45GWBM7-2F-1&_cdi=6766&_user=2073021&_pii=S0012160699994708&_origin=search&_coverDate=11%2F15%2F1999&_sk=997849997&view=c&wchp=dGLzVzb-zSkzS&md5=cc520c758a508e80575a804387429b13&ie=/sdarticle.pdf Dev Biol. 1999 Nov 15;215(2):314-31.] | 1. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. "Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans." [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDG-45GWBM7-2F-1&_cdi=6766&_user=2073021&_pii=S0012160699994708&_origin=search&_coverDate=11%2F15%2F1999&_sk=997849997&view=c&wchp=dGLzVzb-zSkzS&md5=cc520c758a508e80575a804387429b13&ie=/sdarticle.pdf Dev Biol. 1999 Nov 15;215(2):314-31.] |
Latest revision as of 21:23, 25 October 2010
NHR-80 LBD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 232
Design Notes
The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
Source
C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-80 placed into pSB1C3 carrier vector.
References
1. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. "Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans." [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDG-45GWBM7-2F-1&_cdi=6766&_user=2073021&_pii=S0012160699994708&_origin=search&_coverDate=11%2F15%2F1999&_sk=997849997&view=c&wchp=dGLzVzb-zSkzS&md5=cc520c758a508e80575a804387429b13&ie=/sdarticle.pdf Dev Biol. 1999 Nov 15;215(2):314-31.]