Difference between revisions of "Part:BBa K302012:Experience"
RachelBoyd (Talk | contribs) (→Applications of BBa_K302012) |
RachelBoyd (Talk | contribs) (→Applications of BBa_K302012) |
||
| Line 7: | Line 7: | ||
|'''Graph1''': | |'''Graph1''': | ||
|- | |- | ||
| − | |[[Image:Teamnewcastle_yneA168.png|800px]] | + | |[[Image:Teamnewcastle_yneA168.png|800px]][[Image:Teamnewcastle_yneA168BS.jpg|800px]] |
|- | |- | ||
|Graph 1 shows that overexpression of the ''yneA'' gene (Δ''amyE'':pSpac(hy)-oid::''yneA'') leads to a longer cell length compared with our control ''Bacillus subtilis 168''. | |Graph 1 shows that overexpression of the ''yneA'' gene (Δ''amyE'':pSpac(hy)-oid::''yneA'') leads to a longer cell length compared with our control ''Bacillus subtilis 168''. | ||
| Line 13: | Line 13: | ||
|'''Graph2''': | |'''Graph2''': | ||
|- | |- | ||
| − | |[[Image:newcastle_no induction.jpg|600px]] | + | |[[Image:newcastle_no induction.jpg|600px]][[Image:Teamnewcastle_noindBS.jpg|800px]] |
|- | |- | ||
|Graph 2 shows the percentage of cells at different lengths(μm)uninduced | |Graph 2 shows the percentage of cells at different lengths(μm)uninduced | ||
| Line 19: | Line 19: | ||
|'''Graph3''': | |'''Graph3''': | ||
|- | |- | ||
| − | |[[Image:newcastle_0.2 induction.jpg|600px]] | + | |[[Image:newcastle_0.2 induction.jpg|600px]][[Image:Teamnewcastle_0.2indBS.jpg|800px]] |
|- | |- | ||
|Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG | |Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG | ||
| Line 25: | Line 25: | ||
|'''Graph4''': | |'''Graph4''': | ||
|- | |- | ||
| − | |[[Image:newcastle_1IPTG.jpg|600px]] | + | |[[Image:newcastle_1IPTG.jpg|600px]][[Image:Teamnewcastle_1indBS.jpg|800px]] |
|- | |- | ||
|Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG | |Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG | ||
Revision as of 21:16, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K302012
| Graph1: |
 
|
| Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168. |
| Graph2: |
 
|
| Graph 2 shows the percentage of cells at different lengths(μm)uninduced |
| Graph3: |
 
|
| Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG |
| Graph4: |
 
|
| Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG |
Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.
User Reviews
UNIQ29bd7a1394fc6cf1-partinfo-00000000-QINU UNIQ29bd7a1394fc6cf1-partinfo-00000001-QINU