Difference between revisions of "Part:BBa K415301"
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From the '''[http://2010.igem.org/Team:MIT 2010 iGEM Team]'''.
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From the '''[http://2010.igem.org/Team:MIT 2010 MIT iGEM Team]'''.
  
 
This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS.  It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
 
This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS.  It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

Revision as of 21:46, 25 October 2010

Low Power Toggle - Ptrc:cI:tt:Plambda:LacI:tt

The original pTSMa plasmid from the Collins paper. This plasmid has been modified so that it now contains cI that is hypersensitive to cleavage by RecA = Low Power Toggle.


From the [http://2010.igem.org/Team:MIT 2010 MIT iGEM Team].

This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

This part improves upon its predecessor Part:BBa_K415300 in that it requires less UV power to switch states, thus killing fewer cells (see E.Coli death curve).
E.Coli population density as a function of UV exposure. Measured by drip assay.
This plasmid differs genetically from Part:BBa_K415300 in that its inhibitory lambda cI protein is more sensitive to Rec-A cleavage.

The following data was taken from cells co-transformed with K415301 and Part:BBa_K415069.

UV Power Modulation

Photos

(in J/m^2)

Powermod.png
FACS

(in J/m^2)

Facslpt.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]