Difference between revisions of "Part:BBa K415301"

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	|colspan="2"|'''Photos'''		|colspan="2"|'''Photos'''
	(in J/m^2, ignore W)		(in J/m^2, ignore W)
−	|colspan="4"|	+	|colspan="4"|[[Image:powermod.png]]
	|-		|-
	|colspan="2"|'''FACS'''		|colspan="2"|'''FACS'''

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Revision as of 20:43, 25 October 2010

Low Power Toggle - Ptrc:cI:tt:Plambda:LacI:tt

This part is a variation of a [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) from an article in PNAS. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

This part improves upon its predecessor in that it requires less UV power to switch states, thus killing fewer cells (see E.Coli death curve).

The following data was taken from cells co-transformed with K415301 (with and Part:BBa_K415023.

UV Power Modulation
Photos (in J/m^2, ignore W)
FACS (in J/m^2)		[[Image:]]	[[Image:]]	[[Image:]]	[[Image:]]
		0	5	20	40

Sequence and Features