Difference between revisions of "Part:BBa K314300"
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Type VI secretion system from ''Pseudomonas aeruginosa'' in a pCC2Fos CopyControl Fosmid (Chloramphenicol Resistant). | Type VI secretion system from ''Pseudomonas aeruginosa'' in a pCC2Fos CopyControl Fosmid (Chloramphenicol Resistant). | ||
− | + | This page contains the sequence of the original Type VI secretion system (T6SS) fosmid with native ''Pseudomonas aerugginosa'' promoters. This part has been shown to not cause protein expression in ''E. coli'', so it has been deleted. for the working fosmid sequence, go to [[ the recomineered T6SS fosmid https://parts.igem.org/Part:BBa_K314204]] | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 21:01, 27 October 2010
T6SS fosmid
Type VI secretion system from Pseudomonas aeruginosa in a pCC2Fos CopyControl Fosmid (Chloramphenicol Resistant).
This page contains the sequence of the original Type VI secretion system (T6SS) fosmid with native Pseudomonas aerugginosa promoters. This part has been shown to not cause protein expression in E. coli, so it has been deleted. for the working fosmid sequence, go to the recomineered T6SS fosmid https://parts.igem.org/Part:BBa_K314204 Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 332
Illegal EcoRI site found at 2186
Illegal EcoRI site found at 12859
Illegal EcoRI site found at 13560
Illegal EcoRI site found at 20163
Illegal EcoRI site found at 20665
Illegal EcoRI site found at 24358
Illegal XbaI site found at 41958
Illegal XbaI site found at 44779
Illegal SpeI site found at 25862
Illegal SpeI site found at 48309
Illegal PstI site found at 1718
Illegal PstI site found at 4778
Illegal PstI site found at 6473
Illegal PstI site found at 8721
Illegal PstI site found at 8808
Illegal PstI site found at 14226
Illegal PstI site found at 16056 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 332
Illegal EcoRI site found at 2186
Illegal EcoRI site found at 12859
Illegal EcoRI site found at 13560
Illegal EcoRI site found at 20163
Illegal EcoRI site found at 20665
Illegal EcoRI site found at 24358
Illegal NheI site found at 14694
Illegal SpeI site found at 25862
Illegal SpeI site found at 48309
Illegal PstI site found at 1718
Illegal PstI site found at 4778
Illegal PstI site found at 6473
Illegal PstI site found at 8721
Illegal PstI site found at 8808
Illegal PstI site found at 14226
Illegal PstI site found at 16056
Illegal NotI site found at 1
Illegal NotI site found at 847
Illegal NotI site found at 9090
Illegal NotI site found at 10537
Illegal NotI site found at 10700
Illegal NotI site found at 15064
Illegal NotI site found at 22265 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 332
Illegal EcoRI site found at 2186
Illegal EcoRI site found at 12859
Illegal EcoRI site found at 13560
Illegal EcoRI site found at 20163
Illegal EcoRI site found at 20665
Illegal EcoRI site found at 24358
Illegal BglII site found at 13847
Illegal BglII site found at 25843
Illegal BglII site found at 28119
Illegal BglII site found at 28221
Illegal BglII site found at 36179
Illegal BglII site found at 36368
Illegal BglII site found at 37902
Illegal BamHI site found at 353
Illegal BamHI site found at 4845
Illegal BamHI site found at 28473
Illegal BamHI site found at 28851
Illegal BamHI site found at 41336
Illegal BamHI site found at 41952
Illegal XhoI site found at 5951
Illegal XhoI site found at 6482
Illegal XhoI site found at 12346
Illegal XhoI site found at 12739
Illegal XhoI site found at 18729
Illegal XhoI site found at 26791
Illegal XhoI site found at 27404 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 332
Illegal EcoRI site found at 2186
Illegal EcoRI site found at 12859
Illegal EcoRI site found at 13560
Illegal EcoRI site found at 20163
Illegal EcoRI site found at 20665
Illegal EcoRI site found at 24358
Illegal XbaI site found at 41958
Illegal XbaI site found at 44779
Illegal SpeI site found at 25862
Illegal SpeI site found at 48309
Illegal PstI site found at 1718
Illegal PstI site found at 4778
Illegal PstI site found at 6473
Illegal PstI site found at 8721
Illegal PstI site found at 8808
Illegal PstI site found at 14226
Illegal PstI site found at 16056 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 332
Illegal EcoRI site found at 2186
Illegal EcoRI site found at 12859
Illegal EcoRI site found at 13560
Illegal EcoRI site found at 20163
Illegal EcoRI site found at 20665
Illegal EcoRI site found at 24358
Illegal XbaI site found at 41958
Illegal XbaI site found at 44779
Illegal SpeI site found at 25862
Illegal SpeI site found at 48309
Illegal PstI site found at 1718
Illegal PstI site found at 4778
Illegal PstI site found at 6473
Illegal PstI site found at 8721
Illegal PstI site found at 8808
Illegal PstI site found at 14226
Illegal PstI site found at 16056
Illegal NgoMIV site found at 445
Illegal NgoMIV site found at 651
Illegal NgoMIV site found at 716
Illegal NgoMIV site found at 937
Illegal NgoMIV site found at 1528
Illegal NgoMIV site found at 1671
Illegal NgoMIV site found at 1748
Illegal AgeI site found at 1327
Illegal AgeI site found at 3893
Illegal AgeI site found at 10078
Illegal AgeI site found at 15232
Illegal AgeI site found at 23042
Illegal AgeI site found at 25214
Illegal AgeI site found at 25911 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 8802
Illegal BsaI site found at 10084
Illegal BsaI site found at 11635
Illegal BsaI site found at 11681
Illegal BsaI site found at 12017
Illegal BsaI site found at 18711
Illegal BsaI site found at 25528
Illegal BsaI.rc site found at 3890
Illegal BsaI.rc site found at 6326
Illegal BsaI.rc site found at 7457
Illegal BsaI.rc site found at 25664
Illegal BsaI.rc site found at 26422
Illegal BsaI.rc site found at 27692
Illegal BsaI.rc site found at 27893
Illegal SapI site found at 20816
Illegal SapI site found at 46183
Illegal SapI site found at 47393
Illegal SapI.rc site found at 2736
Illegal SapI.rc site found at 5107
Illegal SapI.rc site found at 35683
Illegal SapI.rc site found at 38173
Illegal SapI.rc site found at 38419
Illegal SapI.rc site found at 41177