Difference between revisions of "Part:BBa K364326:Experience"
(→‎Applications of BBa_K364326)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 +
 +
===Ligand===
 +
 +
Ecdysteroids (EcR)
 +
 +
Ecdysteroids  are  insect  moulting  and  sex  hormones  which  include  ecdysone  and
 +
its  homologues  such  as  20-hydroxyecdysone.  Ecdysteroids  also  occur  in  other
 +
invertebrates where they can play a different role. They also appear in many plants
 +
mostly  as  a  protection  agents  (toxins  or  antifeedants)  against  herbivore  insects.
 +
These  phytoecdysteroids  have  medicinal  value  and  are  part  of  herbal  adaptogenic
 +
remedies like cordyceps.
 +
 +
===Physiological functions of EcR===
 +
Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).
 +
 +
===Potential applications of GAL4-EcR LBD===
 +
By the addition of lipid ligands(ecdysteroids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
  
 
===Applications of BBa_K364326===
 
===Applications of BBa_K364326===

Revision as of 01:12, 26 October 2010


Ligand

Ecdysteroids (EcR)

Ecdysteroids are insect moulting and sex hormones which include ecdysone and its homologues such as 20-hydroxyecdysone. Ecdysteroids also occur in other invertebrates where they can play a different role. They also appear in many plants mostly as a protection agents (toxins or antifeedants) against herbivore insects. These phytoecdysteroids have medicinal value and are part of herbal adaptogenic remedies like cordyceps.

Physiological functions of EcR

Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).

Potential applications of GAL4-EcR LBD

By the addition of lipid ligands(ecdysteroids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364326

Picture of gel electrophoresis: Gal4-EcR in pCDNA3.1 resulting an insert of 1142 bp.
Picture of Western blot: Gal4-EcR composite construct is expressed after transferring it into pCDNA3.1

User Reviews

UNIQ09955a8234f9738a-partinfo-00000000-QINU UNIQ09955a8234f9738a-partinfo-00000001-QINU