Difference between revisions of "Part:BBa K325906"

Latest revision as of 16:16, 25 October 2010

pbad CDEG plambda AB

This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon is under control of the arabinose induced pBAD promoter, the second is controlled by the strong constitutive pLambda promoter.

As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 4322
Illegal AgeI site found at 4537
Illegal AgeI site found at 5125
Illegal AgeI site found at 6108
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961
Illegal SapI site found at 6627

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K325906 short</partinfo>
-This part contains two reading frames. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first reading frame is under control of the arabinose induced pBAD promoter, the second is controlled by the strong constitutive pLambda promoter.
+This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon is under control of the arabinose induced pBAD promoter, the second is controlled by the strong constitutive pLambda promoter.
 As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.