Difference between revisions of "Part:BBa K322921"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | SacB promoter is the starting sequence of part BBa K322921. | |
| + | We, SMS_Shenzhen Team, tested the strength of this promoter by comparing it with J23100. | ||
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Revision as of 04:47, 27 October 2017
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.
sacB encodes the Bacillus subtilis levansucrase, which catalyses hydrolysis of sucrose and synthesis of levans (high molecular weight fructose polymers). It is lethal to gram-negative bacteria E-coli.
It works with cat as an alternative method for inserting BioBricks into the genome by using homologous recombination rather than restriction digestion. SacB is used as a negative selection marker, which allows to insert genes onto the chromosomes without leaving a selection marker. The method can thus be reused indefinitely.
The protocol for BRIDGE can be found on the Edinburgh 2010 igem wiki.
http://2010.igem.org/Team:Edinburgh/Project/Protocol
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]