Difference between revisions of "Part:BBa K322705"
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This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method. | This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method. | ||
| − | Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions. | + | Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions. |
This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place). | This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place). | ||
Latest revision as of 15:41, 25 October 2010
E. coli TnaA (tryptophanase) upstream region (500bp)
This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.
Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.
This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]