Difference between revisions of "Part:BBa K422009:Design"
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http://2010.igem.org/Team:ETHZ_Basel
 
http://2010.igem.org/Team:ETHZ_Basel
  
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Neuenschwander, Butz, Heintz1, Kast and Hilvert: A simple selection strategy for evolving highly efficient enzymes. Nature Biotechnology. 2007; 25.
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Neuenschwander, Butz, Heintz, Kast and Hilvert: A simple selection strategy for evolving highly efficient enzymes. Nature Biotechnology. 2007; 25.

Latest revision as of 10:56, 25 October 2010

tetR (AarI A-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 22
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. TetR-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

pKTs

References

http://2010.igem.org/Team:ETHZ_Basel

Neuenschwander, Butz, Heintz, Kast and Hilvert: A simple selection strategy for evolving highly efficient enzymes. Nature Biotechnology. 2007; 25.