Difference between revisions of "Part:BBa K422010:Design"

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===References===
 
===References===
 
http://2010.igem.org/Team:ETHZ_Basel
 
http://2010.igem.org/Team:ETHZ_Basel
 +
Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.

Revision as of 08:50, 25 October 2010

trig (AarI A-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 88


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. Trig-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Escherichia coli genome

References

http://2010.igem.org/Team:ETHZ_Basel Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.