Difference between revisions of "Part:BBa K422011"

 
 
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__NOTOC__
 
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<partinfo>BBa_K422011 short</partinfo>
 
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===Biological Background===
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The prokaryotic actin homologue MreB assembles into helical filaments underneath the cytoplasmic membrane.
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===Cloning strategy===
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BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. '''MreB-C''' is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:51, 25 October 2010

mreB (AarI C-part)

Biological Background

The prokaryotic actin homologue MreB assembles into helical filaments underneath the cytoplasmic membrane.

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. MreB-C is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
    Illegal AgeI site found at 487
    Illegal AgeI site found at 805
    Illegal AgeI site found at 892
  • 1000
    COMPATIBLE WITH RFC[1000]