Difference between revisions of "Part:BBa K305003:Design"

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===Structural Prediction===
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Structural prediction of the protein without the signaling sequence by [http://zhanglab.ccmb.med.umich.edu/I-TASSER/ I-TASSER] yielded the following results. No tertiary structure has been identified by x-ray crystallography or NMR.
  
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===Source===
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[[Image:K305003-chpE-h.gif|200px|frame|structural prediction without signaling sequence]]
  
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codon optimized for B. subtilis
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[[http://zhanglab.ccmb.med.umich.edu/I-TASSER/output/S49452/Full results]].
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 +
===Source===
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Codon optimized for B. subtilis
  
 
===References===
 
===References===
  
 
Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726
 
Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726

Revision as of 14:38, 26 October 2010

Hydrophobic protein chaplin E (chpE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ordered as construct from Mr. Gene after genomic sequence had been identified.


Structural Prediction

Structural prediction of the protein without the signaling sequence by [http://zhanglab.ccmb.med.umich.edu/I-TASSER/ I-TASSER] yielded the following results. No tertiary structure has been identified by x-ray crystallography or NMR.

structural prediction without signaling sequence

http://zhanglab.ccmb.med.umich.edu/I-TASSER/output/S49452/Full results.

Source

Codon optimized for B. subtilis

References

Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726