Difference between revisions of "Part:BBa K404104:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested with XbaI and PstI and we ligated it into the iGEM standard plasmid pSB1C3. | |
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Latest revision as of 03:10, 25 October 2010
p5-TATAless promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 98
Illegal BsaI site found at 135
Design Notes
For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested with XbaI and PstI and we ligated it into the iGEM standard plasmid pSB1C3.
Source
pAAV_RC provided from Stratagene