Difference between revisions of "Part:BBa K364314:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The construction of the LBD, DBD and Backbone have been done with RFC 25.
+
The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
  
 
===Source===
 
===Source===

Revision as of 17:57, 25 October 2010

NHR-8 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 96


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-8 placed into pSB1C3 carrier vector.

References

1. Lindblom TH, Pierce GJ, Sluder AE,"A C. elegans orphan nuclear receptor contributes to xenobiotic resistance" [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-438BMM3-P-1&_cdi=6243&_user=10&_pii=S0960982201002366&_coverDate=06%2F01%2F2001&_sk=%23TOC%236243%232001%23999889988%23253153%23FLA%23display%23Volume_11,_Issue_11,_Pages_R413-R450,_809-908_(1_June_2001)%23tagged%23Volume%23first%3D11%23Issue%23first%3D11%23date%23(1_June_2001)%23&view=c&_gw=y&wchp=dGLbVlW-zSkzV&md5=e1002ea78d04776b5b9ccc020f6c65e5&ie=/sdarticle.pdf Curr Biol. 2001 Jun 5;11(11):864-8.]