Difference between revisions of "Part:BBa K320000"

Revision as of 19:02, 25 October 2010

Asaia replication origin

 This biobrick was obtained by PCR on the pHM2-GFP plasmid (reference). The Asaia that contained this plasmid was provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.

Asaia is a gram negativ bacterium, member of the family of acetic acid bacteria in the alpha-Proteobacteria. [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia] is naturally present in the gut of at least four mosquito species, which are responsible for the transmission of Malaria (Anopheles stephensi, Anopheles gambiae, Aedes aegypti and Aedes albopictus).

 This origin of replication works is Asaia and E.coli. No other bacteria were tested but from [http://www.mobitec.com/int/products/bio/04_vector_sys/pbbr122.html the literature] we know that it works in many gram-negative bacteria.

Sequence and Features Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1160
Illegal AgeI site found at 1320
1000
COMPATIBLE WITH RFC[1000]

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-This sequence was obtain by making a PCR form the GFP plasmid of a straight of Asaia containing this plasmid. This strain has been provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.
+ This biobrick was obtained by PCR on the pHM2-GFP plasmid (reference). The Asaia that contained this plasmid was provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.
-Asaia is a gram negativ bacteria, membre of the family of acetic acid bacterium in the alpha-Proteobacteria.
+Asaia is a gram negativ bacterium, member of the family of acetic acid bacteria in the alpha-Proteobacteria.
+[http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia] is naturally present in the gut of at least four mosquito species, which are responsible for the transmission of Malaria (Anopheles stephensi, Anopheles gambiae, Aedes aegypti and Aedes albopictus).
-Asaia is naturally present in the gut of at leats four strand of mosquitos responsible form the transmission of Malaria (Anopheles stephensi, Anopheles gambiae, Aedes aegypti and Aedes albopictus). The bacteria is transmitted in lateral and vertical way.
+ This origin of replication works is Asaia and E.coli. No other bacteria were tested but from  [http://www.mobitec.com/int/products/bio/04_vector_sys/pbbr122.html  the literature] we know that it works in many gram-negative bacteria.
+Sequence and Features
-This origin of replication works is Asaia and E.Choli. No other bacteria were tested.
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 <span class='h3bb'>Sequence and Features</span>