Difference between revisions of "Part:BBa K364327:Experience"
(→‎Applications of BBa_K364327)
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[[Image:pCDNA3.1-Gal4-ER.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-ER in pCDNA3.1 resulting an insert of 1202 bp.]]
 
[[Image:pCDNA3.1-Gal4-ER.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-ER in pCDNA3.1 resulting an insert of 1202 bp.]]
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===Physiological function of ER===
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In the absence of hormone, estrogen receptors are largely located in the cytosol. Hormone binding to the receptor triggers a number of events starting with migration of the receptor from the cytosol into the nucleus, dimerization of the receptor, and subsequent binding of the receptor dimer to specific sequences of DNA. Some of the effects in humans: Createing proliferative endometrium,breast cell stimulation, increased body fat and weight gain, salt and fluid retention, increased risk of blood clots.
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===Possible applications of GAL4-ER===
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By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
  
 
===Applications of BBa_K364327===
 
===Applications of BBa_K364327===

Revision as of 23:09, 25 October 2010


Picture of gel electrophoresis: Gal4-ER in pCDNA3.1 resulting an insert of 1202 bp.

Physiological function of ER

In the absence of hormone, estrogen receptors are largely located in the cytosol. Hormone binding to the receptor triggers a number of events starting with migration of the receptor from the cytosol into the nucleus, dimerization of the receptor, and subsequent binding of the receptor dimer to specific sequences of DNA. Some of the effects in humans: Createing proliferative endometrium,breast cell stimulation, increased body fat and weight gain, salt and fluid retention, increased risk of blood clots.

Possible applications of GAL4-ER

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364327

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