Difference between revisions of "Part:BBa K313006"
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<partinfo>BBa_K313006 short</partinfo> | <partinfo>BBa_K313006 short</partinfo> | ||
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cDNA of MS2phage (XbaI site is removed) | cDNA of MS2phage (XbaI site is removed) | ||
This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift. | This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift. | ||
It also includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis. | It also includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:27, 24 October 2010
MS2 phage cDNA (XbaI site is removed)
cDNA of MS2phage (XbaI site is removed) This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift. It also includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal BamHI site found at 2057
Illegal XhoI site found at 1048
Illegal XhoI site found at 3214 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 1733
Illegal NgoMIV site found at 2437
Illegal NgoMIV site found at 3309 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1665
Illegal BsaI.rc site found at 163
Illegal BsaI.rc site found at 2257