Difference between revisions of "Part:BBa K323066"
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This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - HivC - Gli1. | This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - HivC - Gli1. | ||
| − | We have bound five chimeric enzymes, encoded by [[Part:BBa_K323135]] and [[Part:BBa_K323132]] to the program sequence and have shown a faster and increased synthesis of violacein. | + | We have bound five chimeric enzymes, encoded by [[Part:BBa_K323135]] and [[Part:BBa_K323132]] to the program sequence and have shown a faster and increased synthesis of violacein, in addition to a decrease in formation of side products, namely deoxychromoviridans. |
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Revision as of 12:54, 25 October 2010
DNA program
This part contains a DNA program - a specifically designed nucleotide sequence containing zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger proteins) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Jazz - Blues - Zif268 - PBSII - HivC - Gli1.
We have bound five chimeric enzymes, encoded by Part:BBa_K323135 and Part:BBa_K323132 to the program sequence and have shown a faster and increased synthesis of violacein, in addition to a decrease in formation of side products, namely deoxychromoviridans.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]