Difference between revisions of "Part:BBa K323131:Design"
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===Source=== | ===Source=== | ||
| − | The ''vio''E sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The PBSII zinc finger is a synthetic gene, which our team designed and ordered from GeneArt. | + | The ''vio''E sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team ([[Part:BBa_K274002]]). The PBSII zinc finger is a synthetic gene, which our team designed and ordered from GeneArt. |
===References=== | ===References=== | ||
Latest revision as of 21:20, 23 October 2010
PBSII_vioE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part:BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
Source
The vioE sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The PBSII zinc finger is a synthetic gene, which our team designed and ordered from GeneArt.