Difference between revisions of "Part:BBa K325905:Experience"

Latest revision as of 00:33, 27 September 2012

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Please enter how you used this part and how it worked out.

Applications of BBa_K325905

User Reviews

[http://2012.igem.org/Team:UC_Chile Team UC_Chile]
We tested the light emittion by this part depending on the addition of two aldehydes substrates: decanal and dodecanal. Our results show that E. coli transformed with this biobrick are unable to emit light when the decanal substrates are added, this can be due to an error in the promoter and/or in the LuxAB proteins. Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates, for more details on the experimental design go to Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].

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UNIQd677108861988df7-partinfo-00000000-QINU UNIQd677108861988df7-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===User Reviews===
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+'''[http://2012.igem.org/Team:UC_Chile Team UC_Chile]'''
+<br>
+We tested the light emittion by this part depending on the addition of two aldehydes substrates: decanal and dodecanal. Our results show that E. coli transformed with this biobrick are unable to emit light when the decanal substrates are added, this can be due to an error in the promoter and/or in the LuxAB proteins.
+Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates, for more details on the experimental design go to ''Luciferase Assay'' at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
+[[Image:lulilulioop.jpg|700px]]
+[[Image:loooliuiop.jpg|700px]]
+|};
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