Difference between revisions of "Part:BBa K325905:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | a | + | This part is a PoPS->light device that needs to be placed under a promoter and induced. It has been assembled from the parts [https://parts.igem.org/Part:BBa_K325902 LuxCD (BBa_K325902)], [https://parts.igem.org/Part:BBa_K325903 LuxEG (BBa_K325903)], a RiPS generator using [https://parts.igem.org/Part:BBa_K081006 plambda (BBa_K081006)] and [https://parts.igem.org/Part:BBa_K216008 LuxAB (BBa_K216008)] using the method of Gibson Assembly. Therefore no BioBrick scars have been created. |
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Latest revision as of 15:55, 25 October 2010
Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3112
Illegal AgeI site found at 3327
Illegal AgeI site found at 3915
Illegal AgeI site found at 4898 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5417
Design Notes
This part is a PoPS->light device that needs to be placed under a promoter and induced. It has been assembled from the parts LuxCD (BBa_K325902), LuxEG (BBa_K325903), a RiPS generator using plambda (BBa_K081006) and LuxAB (BBa_K216008) using the method of Gibson Assembly. Therefore no BioBrick scars have been created.
Source
a