Difference between revisions of "Part:BBa K325906:Design"

 
 
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<partinfo>BBa_K325906 short</partinfo>
 
<partinfo>BBa_K325906 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part will produce light when induced with arabinose. It has been assembled from the parts [https://parts.igem.org/Part:BBa_i0500 pBAD (BBa_i0500)], [https://parts.igem.org/Part:BBa_K325902 LuxCD (BBa_K325902)], [https://parts.igem.org/Part:BBa_K325903 LuxEG (BBa_K325903)], a RiPS generator using [https://parts.igem.org/Part:BBa_K081006 plambda (BBa_K081006)] and [https://parts.igem.org/Part:BBa_K216008 LuxAB (BBa_K216008)] using the method of Gibson Assembly. Therefore no BioBrick scars have been created.
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Latest revision as of 15:53, 25 October 2010

pbad CDEG plambda AB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4322
    Illegal AgeI site found at 4537
    Illegal AgeI site found at 5125
    Illegal AgeI site found at 6108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 6627


Design Notes

This part will produce light when induced with arabinose. It has been assembled from the parts pBAD (BBa_i0500), LuxCD (BBa_K325902), LuxEG (BBa_K325903), a RiPS generator using plambda (BBa_K081006) and LuxAB (BBa_K216008) using the method of Gibson Assembly. Therefore no BioBrick scars have been created.


Source

a

References