Difference between revisions of "Part:BBa K325229"

 
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{{:Templates/Cambridge-fireflycolour|colour=green|substitution=Val239Ile}}
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__NOTOC__
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<partinfo>BBa_K325229 short</partinfo>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K325229 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K325229 parameters</partinfo>
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Revision as of 02:53, 27 October 2010

Input: L-Arabinose
Output: Light (green)

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     [[Part:BBa_K325{{{id}}}:Experience |Add Data]]       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3