Difference between revisions of "Part:BBa K323131:Design"

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===Design Notes===
 
===Design Notes===
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The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.
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The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site ([[Part:BBa_B0034]]). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon ([[Part:BBa_K274002]]) and ligated as a back insert into the RBS_zinc-finger construct.
  
 
===Source===
 
===Source===

Revision as of 21:19, 23 October 2010

PBSII_vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PBSII zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioE gene was obtained by PCR from an existing BioBrick of violacein operon (Part:BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

The vioE sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The PBSII zinc finger is a synthetic gene, which our team designed and ordered from GeneArt.

References