Difference between revisions of "Part:BBa K330000:Experience"

Revision as of 15:53, 23 October 2010 (view source) Hanson (Talk | contribs) (→‎Applications of BBa_K330000) ← Older edit		Latest revision as of 17:42, 23 October 2010 (view source) Hanson (Talk | contribs) (→‎Applications of BBa_K330000)
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	===Applications of BBa_K330000===		===Applications of BBa_K330000===
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		+	Protein tag
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		+	===Characterization of BBa_K330000===

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Latest revision as of 17:42, 23 October 2010

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Applications of BBa_K330000

Protein tag

Characterization of BBa_K330000

To demonstrate that this biobirck, “FLAG-tag encoding sequence flanked by standard BioBrick™ prefix and suffix”, works as expected, a construct FLAGtag – EGFP was built and ligated into mammalian expression vector pUHD-P3. HtTA1 cells were transfected with this plasmid and observed under florescence microscope 48 hours after transfection. The cells were then lysed and protein extracts obtained. The extracted proteins were then subjected to SDS-PAGE and blotted with M2 (Sigma, anti-flagtag). HtTA1 cells transfected with empty pUHD-P3 were used as control.

The results are as follows: