Difference between revisions of "Part:BBa K364202"

Revision as of 07:44, 24 October 2010

hBAX

Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).

Picture of gel electrophoresis: hBAX in pSB1C3 resulting an insert of 573 bp. Circular pSB1C3-hBAX and restricted PMAT-hBAX can be seen as control on the gel.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 449
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K364202 short</partinfo>
-Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis which is the most frequent type of PCD (Programmed cell death).
+Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).
 [[Image:Team_Debrecen_20101008.jpg|800px|thumb|center|Picture of gel electrophoresis: hBAX in pSB1C3 resulting an insert of 573 bp. Circular pSB1C3-hBAX and restricted PMAT-hBAX can be seen as control on the gel.]]