Difference between revisions of "Part:BBa K313017"

 
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<partinfo>BBa_K313017 short</partinfo>
 
<partinfo>BBa_K313017 short</partinfo>
  
This is a device that aims to measure the leakiness of terminator BBa_B1006.
+
This is a device that aims to measure the leakiness of a terminator BBa_B1006.
 +
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
 +
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:34, 27 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006. If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]