Difference between revisions of "Part:BBa K313016"
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<partinfo>BBa_K313016 short</partinfo> | <partinfo>BBa_K313016 short</partinfo> | ||
| − | + | This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator." | |
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| + | lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. | ||
| + | So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre. | ||
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Revision as of 13:14, 26 October 2010
cre recombinase assay device
This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."
lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1139
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 993
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 439
Illegal NgoMIV site found at 599
Illegal NgoMIV site found at 967 - 1000COMPATIBLE WITH RFC[1000]