Difference between revisions of "Part:BBa K404106:Design"

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===Design Notes===
 
===Design Notes===
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The phTERT was created by PCR amplifying the phTERT with overhanging RFC10 iGEM restriction sites and ligated it into pSB1C3. The pHTERT does not contain any iGEM restriction sites being compatible with the RFC25 and RFC10 standard.
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===Source===
 
===Source===

Revision as of 23:20, 22 October 2010

phTERT promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phTERT was created by PCR amplifying the phTERT with overhanging RFC10 iGEM restriction sites and ligated it into pSB1C3. The pHTERT does not contain any iGEM restriction sites being compatible with the RFC25 and RFC10 standard.

Source

x

References

Cech, T., 2000. Life at the End of the Chromosome: Telomeres and Telomerase. Angewandte Chemie (International ed. in English), 39(1), 34-43. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10649348.
Kyo, S. et al., 2008. Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers. Cancer science, 99(8), 1528-38. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18754863.
Maida, Y. et al., 2002. Direct activation of telomerase by EGF through Ets-mediated transactivation of TERT via MAP kinase signaling pathway. Oncogene, 21(26), 4071-9. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12037663.
Nishi, H. et al., 2004. Hypoxia-inducible factor 1 mediates upregulation of telomerase (hTERT). Molecular and cellular biology, 24(13), 6076-83.
Takakura, M. et al., 2005. Function of AP-1 in Transcription of the Telomerase Reverse Transcriptase Gene ( TERT ) in Human and Mouse Cells. Society, 25(18), 8037-8043.
Wick, M., Zubov, D. & Hagen, G., 1999. Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT). Gene, 232(1), 97-106. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10333526.