Difference between revisions of "Part:BBa K314015"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:44, 22 October 2010
CapD_CP-F24H
A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.
To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1489
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]