Difference between revisions of "Part:BBa K349007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part has built-in BsaI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method. | |
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===Source=== | ===Source=== | ||
Latest revision as of 02:51, 27 October 2010
KanR-AB-BsaI (for construction of AB BioBytes 2.0)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 984
Design Notes
This part has built-in BsaI cutsites surrounding the p1003 kanamycin resistance cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.
Source
pSB1C3