Difference between revisions of "Part:BBa K314012"

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A circularly permuted CapD using a Foldit-designed linker
 
A circularly permuted CapD using a Foldit-designed linker
  
To test BBa _K314012, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD_CP was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity, for a detailed description of the assay please see the http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki].  The resulting data is shown below.
+
To test BBa _K314012, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD_CP was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki].  The resulting data is shown below.
  
 
<gallery heights=400px widths=350>
 
<gallery heights=400px widths=350>

Revision as of 23:03, 21 October 2010

CapD_CP

A circularly permuted CapD using a Foldit-designed linker

To test BBa _K314012, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD_CP was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]