Difference between revisions of "Part:BBa K337008"
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<partinfo>BBa_K337008 short</partinfo> | <partinfo>BBa_K337008 short</partinfo> | ||
| − | hsa-miR-122 binding site pattern, consisting of 2 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites. | + | hsa-miR-122 binding site pattern, consisting of 2 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites (BB-2, RFC12). |
The binding sites were created by using oligos where the reverse complemented nucleotides 9-12 of the corresponding miRNA were randomised: | The binding sites were created by using oligos where the reverse complemented nucleotides 9-12 of the corresponding miRNA were randomised: | ||
Revision as of 09:38, 27 October 2010
hsa-miR-122 Binding site pattern (2BS) with randomised nt9-12
hsa-miR-122 binding site pattern, consisting of 2 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites (BB-2, RFC12).
The binding sites were created by using oligos where the reverse complemented nucleotides 9-12 of the corresponding miRNA were randomised:
- CAAACACCATNNNNACACTCCA
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]