Difference between revisions of "Part:BBa K314100"
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<partinfo>BBa_K314100 short</partinfo>
 
<partinfo>BBa_K314100 short</partinfo>
  
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High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the standard Elowitz standard RBS B0034.  When digested and ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.  
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High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the Elowitz standard RBS B0034.  When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:56, 21 October 2010

High constitutive expression cassette

High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]