Difference between revisions of "Part:BBa K314200:Design"
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Cell Host Microbe.�2010 Jan 21;7(1):25-37 PMID: 20114026 | Cell Host Microbe.�2010 Jan 21;7(1):25-37 PMID: 20114026 | ||
Revision as of 00:00, 21 October 2010
Toxin Tse2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 196
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 378 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 with the F2620 promoter.
Source
Pseudomonas aeruginosa
References
Cell Host Microbe.�2010 Jan 21;7(1):25-37 PMID: 20114026